FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097806
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCT16500.064.696633
GTAATAG18450.064.308615
GCGCTAG13100.063.5874565
CGCTAGC9800.063.213966
GGCGCTA17600.063.0394484
AGGTAAT19500.062.9996763
CGCTAGA15200.062.8615156
CGCTAAA14450.062.4910246
TGCGCTA9250.062.053734
GGATAAT25250.061.821463
GCGCTAT14550.061.8209845
CCGCTAG13050.061.6855055
TCGCTAG13750.061.5996785
CGCTCAT11650.061.587666
GTGTAAT10400.061.586223
CCGCTAT15600.061.4740375
CGCTAGT12500.061.3196756
CGCTACT9750.061.0253266
AGCGCTA11900.060.882044
TAATAGT13400.060.59676
Produced by FastQC (version 0.11.5)