FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556073
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG5700.066.929745
GCGCTAT8000.065.1874165
CGCTAGC4750.064.8420266
CGCTAGA8700.064.770036
GGGCGCT8750.063.999923
CCGCTAG6950.063.9567535
CCGCTAT8650.063.525935
CGCTAGT6050.063.057776
TCGCTAG7200.062.7082565
GGCGCTA9400.062.5531164
CGCTATT8400.062.4999246
TGCGCTA5000.062.299924
GCCGCTA7050.061.56024
GCGCTCT8550.061.4034275
GTAATAG7250.061.310275
GATAATC7900.061.139164
CCCGCTA6300.061.1110344
AGCGCTA5100.061.0783584
GAGTAAT12200.060.819593
AGTAATC9050.060.718154
Produced by FastQC (version 0.11.5)