FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454285
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT7650.064.50985
GACGCTA3150.064.444444
ACGCTAT4050.063.9506155
CGCTATT7850.063.757966
TCGCTAG5450.063.5779765
GGGCGCT6350.063.385833
GCGCTAG4700.063.2978745
CGCTAGT4600.063.1521726
TGCGCTA4050.063.086424
CGCTAGA7500.063.0000046
AGCGCTA5200.062.5961534
GTCCGCT4550.062.307693
CGCTCTA4500.062.222226
GCCCGCT4450.062.134833
CGCTATA7950.062.075476
CGCTAAA5550.061.80186
CGCTATG5500.061.727276
TACGCTA2850.061.4035074
GGATAAT10900.061.330273
GGCGCTA7900.061.1392444
Produced by FastQC (version 0.11.5)