FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_UB_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405537
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG4200.066.6664355
CGCTAGT4900.063.57126
CGCTAGA5950.063.5291866
GCGCTAT4900.062.856925
GCGCTAG4350.062.75845
CGCTATT5250.061.9997836
GGGCGCT6050.061.9006083
GGCGCTA5950.061.764494
GTAATGT5050.061.682955
TCGCTAG5450.061.6511575
AGCGCTA4600.061.6302154
TGCGCTA3650.061.3696444
GCGCTCT4700.061.0636185
GTAATAG6200.060.967535
CGCTAAA5900.060.5082636
CGCTATG4400.060.454336
CGCTAGG3200.060.1560366
GGATAAT7600.059.8682063
ATCGCTA6200.059.83854
AGTCGCT2400.059.791463
Produced by FastQC (version 0.11.5)