FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7842
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG120.1530221882172915TruSeq Adapter, Index 1 (100% over 49bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA100.12751849018107625TruSeq Adapter, Index 1 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCCT150.002142751870.03
GAGCGCT308.9736204E-870.03
GCTAGAA150.002142751870.07
TCCTAGA150.002142751870.06
CCGCTAG252.5852623E-670.05
CGCTAGA550.070.06
CGCTACC150.002142751870.06
GTCCTAG150.002142751870.05
AGAGCGC150.002142751870.02
AGTAATC207.446116E-570.04
GCGCTTG150.002142751870.05
GAGTAAT150.002142751870.03
TAATTTA308.9736204E-870.06
GCGCTAT150.002142751870.05
GCGCTAG207.446116E-570.05
GCCGCTA150.002142751870.04
AGCGCTT150.002142751870.04
AGCGCTC150.002142751870.04
AGCGCTA252.5852623E-670.04
AGGTAAT252.5852623E-670.03
Produced by FastQC (version 0.11.5)