FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50491
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA6831.3527163256818047TruSeq Adapter, Index 1 (100% over 40bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA3300.6535818264641223TruSeq Adapter, Index 1 (100% over 40bp)
GGCGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1750.34659642312491334TruSeq Adapter, Index 1 (100% over 40bp)
GGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1140.225782812778515TruSeq Adapter, Index 1 (100% over 40bp)
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGC790.15646352815353232TruSeq Adapter, Index 1 (97% over 39bp)
TGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA790.15646352815353232TruSeq Adapter, Index 1 (100% over 40bp)
GGGACTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA620.12279416133568358TruSeq Adapter, Index 1 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG600.11883305935711315TruSeq Adapter, Index 1 (100% over 49bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA590.11685250836782793TruSeq Adapter, Index 1 (100% over 37bp)
GAGCGCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG590.11685250836782793TruSeq Adapter, Index 1 (100% over 39bp)
GAGCGCGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG580.11487195737854271TruSeq Adapter, Index 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA455.456968E-1270.000016
AGCGCTA650.070.000014
GTGTAAT455.456968E-1270.000013
TAATCAA401.3096724E-1070.06
CTTAATT150.002212561270.04
ATTTACC207.855486E-570.08
ATCCTGA150.002212561270.05
ATCCTCA301.0045551E-770.05
GGGGATA301.0045551E-770.01
TAATAAC401.3096724E-1070.06
CTCACGT150.002212561270.08
CCTTAAT150.002212561270.03
AGTCCGC207.855486E-570.02
GATAATA353.61797E-970.04
AATTTAC550.070.07
GGTAATA500.070.04
TTAATGT150.002212561270.05
CTACTAG150.002212561270.08
GGGCTCC207.855486E-570.02
GCGGTCC150.002212561270.02
Produced by FastQC (version 0.11.5)