FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18395
Sequences flagged as poor quality0
Sequence length76
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA3692.0059798858385434TruSeq Adapter, Index 1 (100% over 40bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA2141.1633596085892906TruSeq Adapter, Index 1 (100% over 40bp)
GGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1160.6306061429736342TruSeq Adapter, Index 1 (100% over 40bp)
GGCGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1130.6142973634139711TruSeq Adapter, Index 1 (100% over 40bp)
TGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA590.32073933134003807TruSeq Adapter, Index 1 (100% over 40bp)
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGC430.2337591736885023TruSeq Adapter, Index 1 (97% over 39bp)
AGGGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA380.2065778744223974TruSeq Adapter, Index 1 (100% over 40bp)
GGGACTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA350.19026909486273444TruSeq Adapter, Index 1 (100% over 40bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA290.15765153574340854TruSeq Adapter, Index 1 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG260.1413427561837456TruSeq Adapter, Index 1 (100% over 49bp)
AGGAGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA260.1413427561837456TruSeq Adapter, Index 1 (100% over 40bp)
GAGCGCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG240.13047023647730363TruSeq Adapter, Index 1 (100% over 39bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGC220.11959771677086165TruSeq Adapter, Index 1 (97% over 39bp)
AGTGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA200.10872519706441967TruSeq Adapter, Index 1 (100% over 40bp)
AGGGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA190.1032889372111987TruSeq Adapter, Index 1 (100% over 40bp)
TGCGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA190.1032889372111987TruSeq Adapter, Index 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG500.070.000015
AATTTAG252.731109E-670.000017
GGGTAAT252.731109E-670.000013
CCGCTAG500.070.000015
GATAATT500.070.000014
AATTACA252.731109E-670.000017
GGATAAT550.070.000013
CTAATTA252.731109E-670.000015
GCGCTCA252.731109E-670.000015
GGCTAAT252.731109E-670.000013
CGTCGCT500.070.000013
GCCGCTA550.070.000014
GAATAAT252.731109E-670.000013
GGTGGAA252.731109E-670.0000170
TGGCGCT252.731109E-670.000013
CGTGAAA252.731109E-670.0000170
GTAATTA252.731109E-670.000015
GGGTGGA252.731109E-670.0000169
AGATAAT252.731109E-670.000013
GGACGCT252.731109E-670.000013
Produced by FastQC (version 0.11.5)