FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22114
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG420.1899249344306774TruSeq Adapter, Index 1 (100% over 49bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA330.14922673419553223TruSeq Adapter, Index 1 (100% over 37bp)
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA260.11757257845708602TruSeq Adapter, Index 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC150.002196064570.05
ATCCTGA150.002196064570.05
CGCTGGA150.002196064570.06
GAGGTCC150.002196064570.02
GAGCGCT550.070.03
CGATAAT150.002196064570.03
TGCGCTA252.7498281E-670.04
AAATCCT150.002196064570.03
GTCGCTC252.7498281E-670.04
CGGGGTA150.002196064570.01
GGCGCTC150.002196064570.04
TGGTAAT353.4942786E-970.03
AATTTAT353.4942786E-970.07
TGCCGCT150.002196064570.03
CCGCTAT252.7498281E-670.05
GATAATT309.785617E-870.04
TATCGCT252.7498281E-670.03
GTATCCT150.002196064570.03
CCTGACC150.002196064570.07
CGCTATG252.7498281E-670.06
Produced by FastQC (version 0.11.5)