FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9962
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA230.23087733386870107TruSeq Adapter, Index 1 (100% over 40bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA210.2108010439670749TruSeq Adapter, Index 1 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG200.20076289901626182TruSeq Adapter, Index 1 (100% over 49bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA200.20076289901626182TruSeq Adapter, Index 1 (100% over 40bp)
AGGAGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA140.14053402931138326TruSeq Adapter, Index 1 (100% over 40bp)
TGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA110.11041959445894399TruSeq Adapter, Index 1 (100% over 40bp)
GGGACTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA110.11041959445894399TruSeq Adapter, Index 1 (100% over 40bp)
GGCGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA100.10038144950813091TruSeq Adapter, Index 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG453.6379788E-1270.000015
ATTAATT252.639379E-670.000014
GCCGCTA453.6379788E-1270.000014
AGCGCTG252.639379E-670.000014
GGAATAA252.639379E-670.000012
GGAGCGC252.639379E-670.000012
CGCCGCT252.639379E-670.000013
ACTAATT150.002160468470.04
GAGCGCT353.2359821E-970.03
CACATAA150.002160468470.041
TGGTAAT150.002160468470.03
GGTCCTG150.002160468470.04
GGCGCCG207.549324E-570.01
GGGTAAT401.1277734E-1070.03
TATAATA150.002160468470.04
GGCCGCT150.002160468470.03
GATAATT150.002160468470.04
GATAATG150.002160468470.04
AGGGTAA207.549324E-570.02
AATTACA207.549324E-570.07
Produced by FastQC (version 0.11.5)