FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135594
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG24000.2113431384808303TruSeq Adapter, Index 1 (100% over 49bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA18030.15877153278372375TruSeq Adapter, Index 1 (100% over 37bp)
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA17790.15665810139891545TruSeq Adapter, Index 1 (100% over 40bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA11670.10276560108630373TruSeq Adapter, Index 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA48500.068.5566566
CCGCTAG24400.067.417995
GCGCTAG22700.066.762085
GAGCGCT21200.066.6980743
AGCGCTA14300.065.839124
CGCTAGC7150.065.594376
TAATTTA37900.065.3825456
TCGCTAG12650.065.29645
GCCGCTA26500.064.9810944
GCGCTAT11850.064.978865
AGCGCTC15300.064.738524
GGCGCTA27100.064.446454
GGGCGCT18100.064.198853
GTAATAC8600.063.4883355
CGCTATT9300.063.2257656
ACGCTAG10150.063.1034055
GGTAATT23850.063.1026884
GCTAGAT45000.062.999967
AGGTAAT18250.062.9040683
TAATTAC18650.062.8685876
Produced by FastQC (version 0.11.5)