FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548048
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG15640.28537646337547073TruSeq Adapter, Index 1 (100% over 49bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA13520.24669372025807956TruSeq Adapter, Index 1 (100% over 37bp)
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA10150.18520275596298133TruSeq Adapter, Index 1 (100% over 40bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA7290.13301754590838757TruSeq Adapter, Index 1 (100% over 40bp)
GGGACTCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA7040.12845590167284618TruSeq Adapter, Index 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA32650.068.177116
TAATTTA25250.067.2271966
GTAATTT11050.066.838165
GCCGCTA12850.066.464694
GCGCTAG14100.066.282135
CCGCTAG14000.066.255525
AGCGCTT5250.066.005514
GCGCTAT5150.065.927835
GAGCGCT12250.065.719773
AAGCGCT8100.065.6844943
TAATTAC13400.065.559186
GGCGCTA14200.065.322354
GGTAATT12350.064.9042054
AGCGCTA7800.064.620784
AGCGCTC10750.064.470494
GACGCTA6150.064.314314
GATAATT9550.064.141474
GGGCGCT8200.064.029733
GGGTAAT9550.063.7749523
GCTAGAT31550.063.3434077
Produced by FastQC (version 0.11.5)