FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324045
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG45280.34198233443727366TruSeq Adapter, Index 1 (100% over 49bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA22300.16842327866500004TruSeq Adapter, Index 1 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTA15750.067.1128544
GCGCTAG20050.066.685025
CGCTAGA51700.066.6168066
GCGCTAT11400.066.3175055
CCGCTAG26700.066.200225
TAATTTA48650.065.397386
GGGCGCT18600.064.9210363
GAGCGCT25300.064.6064153
GGGTCCT12000.064.460013
TAATTAC27400.064.253496
GCCGCTA25150.064.156734
AAGCGCT18600.063.7919773
TGCGCTA7850.063.7596134
GCGCTAA13450.063.496075
GTAATAC9550.063.404785
GGTAATT26050.063.2837874
GTAATTT23850.062.66415
TAGCGCT9900.062.577383
GATAATT22300.062.311034
GGTCCTA9550.062.3052754
Produced by FastQC (version 0.11.5)