FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_pool190111A_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213619
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG51560.4248450296180268TruSeq Adapter, Index 1 (100% over 49bp)
GTAAGCGCTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA27830.22931414224727859TruSeq Adapter, Index 1 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA58450.067.065836
GCGCTAG20300.066.2068565
TAATTTA63250.066.181786
CCGCTAG27500.066.0545045
AGCGCTA17150.065.918334
AAGCGCT19400.065.4896553
GAGCGCT27800.065.467583
TCGCTAG13450.065.315955
GCCGCTA26550.064.990544
GTAATTT27800.064.838095
TAATTAC35500.064.774616
GGGCGCT15300.064.509773
GCGCTAT10600.064.386765
GTTAATT18850.063.1299364
GGGTCCT11700.063.119623
GGCGCTA20050.063.0174184
AGCGCTC20500.062.999964
GGATAAT17950.062.9804653
GGTAATT27650.062.9113544
AGCGCTT14300.062.902064
Produced by FastQC (version 0.11.5)