FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Ler_B_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Ler_B_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626167
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC5450.017.33929870
CACGTCG2354.5684963E-513.40413870
GTCACTG13050.011.53246669
CGTCTGC3953.5653466E-711.51888770
GCGTGCG2802.3447693E-411.25024748
GTGAGGG2250.00670306810.88879320
CACTGAC8650.010.11551970
CGTGCGG3259.267407E-49.69252149
AGTCACT15600.09.64735168
TCCAGTC20750.09.445770
CGGAAGC9051.8189894E-129.28168770
CAGTCAC17350.08.87600267
TCACTGA13150.08.78319470
CGCGTGC3700.00301857628.513700547
CTCCAGT24300.08.49786869
CCAGTCA17550.08.37599566
TCACTGC4753.8283702E-48.10519270
ACTCCAG25750.08.01934768
ACTGACC7502.587718E-67.466601469
CAGGCGT4900.0046286457.14279417
Produced by FastQC (version 0.11.5)