FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Ler_B_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Ler_B_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069518
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG1452.4391958E-416.89648470
CACACGG1906.121196E-616.5788870
GGGCGTG1550.00737417613.54833210
AAGAGCC5600.013.12494770
GAGCACC3701.523349E-712.29724870
CTGACCA6300.012.22217370
ACTGACC6700.012.01487769
TCACTGC3208.0364966E-49.8437170
CAGTCAC15300.09.83656270
CACTGAC8051.4551915E-119.56517968
CCAGTCA16500.09.33329569
GTCACTG11050.09.18548470
CGGAAGC6503.0388037E-89.1538170
AGTCACT13700.08.43062370
CTCCAGT21500.07.97671270
TCCAGTC18700.07.860930468
ACTCCAG22550.07.294871369
GAACTCC27950.07.262940469
AACTCCA28000.07.12497170
TGAACTC34650.06.96966970
Produced by FastQC (version 0.11.5)