FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Ler_B_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Ler_B_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292804
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACC3458.743191E-712.17404670
CTGACCA12200.011.47553569
CACTGCC3702.515372E-510.40551970
ACTGACC14100.010.17741568
TCACTGC6351.9790605E-99.92136970
CACACGG5201.1827797E-69.4231870
AGTCACG4158.862726E-59.27720970
CACTGAC16050.08.940907567
CCAGTCC4401.6749455E-48.75009670
AGCCCAC4250.001145258.23538567
CAGTCAC30000.08.16675669
GTCACTG22750.07.846239669
AGTCACT28400.07.764168770
TCCAGTC36150.07.648769470
CCAGTCA31750.07.38590768
GATCGCA5350.00136436597.196340668
AAGAGCC9351.8206629E-77.11237770
GAACTCC55450.07.006388769
CTCCAGT41700.06.96650369
AACTCCA56500.06.690338670
Produced by FastQC (version 0.11.5)