FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Ler_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Ler_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955804
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGG5550.019.5495370
GAGCACC8550.013.50875870
ACTGACC18500.012.86485370
CACTGAC21250.011.36469569
TCACTGC7201.8189894E-1210.69443470
CAGTCAC43700.010.652163570
CTGACCA16550.010.57400770
CTGAACG3401.2273715E-410.29410770
CCAGTCA48350.09.62770669
CACGTCG6008.7233275E-89.33332470
GTCACTG31200.09.31088870
AGTCACT38900.09.08739670
AAGAGCC14350.08.780478570
CTCCAGT63350.08.67402570
TGACTGG4956.4320775E-58.4848430
TCCAGTC53850.08.449388568
TCACTGA30400.08.17433568
CGCGAGG4300.0012861178.13952717
ACTCCAG72250.07.993071669
AGTCACG4500.00201334277.777770570
Produced by FastQC (version 0.11.5)