FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_input_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_input_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448031
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCCG1000.00969155117.552
GGTGACC1503.1598477E-416.33333418
TGCAGGG2356.8482745E-411.91489438
CCTACTG2150.004777495311.3953491
GTGACCT2700.002184932610.3703719
CCCAATG3900.00485403848.0769231
CTTATGA7806.652226E-88.0769231
TATGCCT5301.4242459E-47.92452762
CTCTTAC5502.1870699E-47.6363641
CTTACTA5552.4279793E-47.56756731
CTTAGAG4800.00378534347.29166651
CTATGAT8251.388993E-67.2121211
CTTAGTT7301.4160909E-57.19178061
CTATATA23000.07.1521741
CTTAACT7056.945224E-56.9503541
CTACTAT6259.461582E-46.72000031
CTATACT6259.461582E-46.72000031
CTTCCCA5250.0090271466.66666651
CGCAACC10351.0587664E-66.42512146
CCTAAAC6550.00160857276.4122141
Produced by FastQC (version 0.11.5)