FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_input_e-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_input_e-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914312
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGT500.00720960928.00000267
TGTGACG1200.001340783617.50000268
TACAGGG1000.00968889917.50000237
GTGCTGT1400.0037531415.070
GGCCAAT1450.00473892114.482758518
CATAGGC1500.005934010714.020
CTTGCGA1600.00909420913.12561
TGCGATT2203.9277624E-412.72727427
CTATTCG2100.004006011411.6666671
CTCATGT5703.5397534E-910.43859613
TCTCATG5451.963781E-810.27522912
TATGACT12350.08.7854252
GACTTGA10303.274181E-118.1553395
GCAACCC4350.00143932288.04597847
ACCCGAT4400.0016110937.95454650
CGCAACC7503.0488263E-77.933333446
ATGACTT12900.07.8682173
TCATGTG13800.07.862318514
CATGTGT14900.07.751677515
TGACTTG12505.456968E-127.564
Produced by FastQC (version 0.11.5)