FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_input_e-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_input_e-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635619
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCG1350.002947386115.5555017
GACATCC2500.001150428611.19996116
GAGCACC3309.065638E-510.60602470
TGATGTC15950.08.9968359
ATATGAC22850.08.88480951
TGTCATG19400.08.65976412
CTTGATG18750.08.3999717
TATGACT23850.08.0714972
ATGACTT24000.07.87521363
GTCATGT19350.07.7777513
GATGTCA17800.07.66851210
TGACTTG21300.07.55889034
CCGATCT8958.452298E-87.430141452
GAGTATA26100.07.24135428
GACTTGA19400.07.21669055
CTTCATG8251.3876943E-67.2127581
CCCGATC8754.5744855E-77.199975551
ACTACCA5000.0056323446.99997536
ATGTCAT21250.06.91762311
GGAGTCG14301.8189894E-116.853122763
Produced by FastQC (version 0.11.5)