FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_input_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_input_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990273
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGC1350.002947696515.55549446
GTCACCG2001.754805E-413.99994470
CGACTCG2408.172855E-411.6669146
GGGTCCC2100.004008964611.6666240
CCGTTCT3000.00522587089.33329616
TGGGTCC3150.0078029058.88885439
ATATGCG4502.1355605E-48.5559511
CTCACAG4159.040941E-48.4341251
ATTAGGG6452.1455016E-57.59725051
ATACCCT4900.00462725347.14318751
TGACTTG25600.07.1097044
CTATGCT4950.005107337.0710341
CCAAGCA5700.0026636846.75435917
TTAACGT5750.00291964256.6956268
GCTATGA5250.0090309716.666640359
CTATATG11803.470086E-86.5257261
GACTTGA23650.06.5119295
TATGACT28650.06.47499472
CGCAACC16853.6379788E-126.439143746
CACCTTG18000.06.41664168
Produced by FastQC (version 0.11.5)