FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e3-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e3-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184592
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGAC350.001253175239.9990738
AATGTCG400.00241446834.99918461
GTAGTTC500.007195756327.9993555
TAATCTA1950.002288042812.56381117
CTACACC2550.001347773510.98013768
ATATACA2650.001853206910.5686551
GACTCAT3200.008815518.74979650
AAGACTC3800.00380970148.28928148
GTTAGAA3800.00380970148.28928129
ATTAATA3900.0048027298.0789241
ATGTTTT4050.00674019947.77970464
ATATAAT4600.0024653677.61058041
Produced by FastQC (version 0.11.5)