FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e3-1h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e3-1h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128798
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATGT200.00693809552.565
TACTACC350.001251620740.08
TAGAGGA400.0024114935.042
TCGAACC400.0024114935.034
ATTTCCT1000.00964509817.51
GAGTCTT1850.00153524713.2432435
AGTCTTT2200.00562278311.1363636
ACAAAGC2850.00338512689.82456239
Produced by FastQC (version 0.11.5)