FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e3-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e3-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611943
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC750.001832055323.33333434
GAGGGCA800.002665735821.87510
TGCAGGC1000.00968534817.514
CAACCGG2150.0047721311.39534948
CTATATG3451.1563101E-511.159421
GCGTAAG3652.1577811E-510.54794564
CCGGATC2700.002181838310.3703751
GGCGTAA3401.2237314E-410.29411863
CGCAACC5805.135371E-89.65517246
AACCGCA8801.8189894E-129.54545443
AGGCGTA3702.86931E-49.45945962
ACCGCAA8001.2914825E-109.187544
CGTAAGA4351.4750114E-48.850574565
AAGGCGT4006.257199E-48.7561
CCGCAAC7604.5911293E-98.7545
AAACCGC8252.215529E-98.48484842
GAGTATA11150.08.47533628
AAAGGCG4350.00143764188.04597760
TAAAGGC5006.6186354E-47.700000359
TAAACCG9101.3800673E-87.69230841
Produced by FastQC (version 0.11.5)