FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e3-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e3-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587235
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGG400.002418487634.99995820
CCGGTTG1003.9305023E-420.99997510
CGGTTGC1708.166785E-414.41174711
CGTTATA1850.001546665113.24322716
GATCCGG1600.00908921213.1249847
CTTTAAG5805.1266397E-89.6559831
GCACACG3350.00122068869.40297370
CTTATCT3750.0033995218.4007051
AGCACAC3850.00431394348.18180869
TGTAGCC4905.330374E-47.857133417
GATCTCA4050.00679955967.77776810
GAGCACA4100.00758904037.682917668
GTAGCCA5057.3600665E-47.62375318
CTTAATC4150.0084511727.5909991
TCTCATG4150.0084576067.590352512
TGACTTG7402.1034557E-67.56755834
AGAGCAC4650.00276949687.52687367
CCGCAAC6201.0783697E-47.338700345
GACTTGA7251.279774E-57.241375
ATCTTGA5450.00165344047.0648131
Produced by FastQC (version 0.11.5)