FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e2-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e2-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294162
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCGG450.004308754631.11097170
TGAGCCG1500.005935941413.99993912
CTTAAGG3055.168166E-410.3282221
CTATTAG4905.657683E-69.2860331
CTCTATT8951.1787051E-98.2125711
CTATTAA11051.8189894E-117.9188231
CATAAGG4550.00224138827.6925711
CTATACA6804.3149994E-57.20612861
CTCTATA8501.6600123E-56.5884611
CTCTTAA7451.4280544E-46.57740641
CTCAATG6450.00135118356.51185041
CTATTGA6500.00147437936.46175961
CTTTAAT13551.882654E-96.45778561
CTTAGGC13802.8303475E-96.340551443
CTATATG8853.001692E-56.32790041
ACTTAGG13308.563802E-96.315761642
CTCATTC6200.0064057056.209891
AAGGCGT7358.645575E-46.1904495
CTATGTA8053.8923652E-46.0871651
CTTTACA8053.8923652E-46.0871651
Produced by FastQC (version 0.11.5)