FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e2-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e2-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724016
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG350.001255461140.04
GACAGGG500.007208634627.99999868
GCAAGCG500.007208634627.99999848
CGATTGC1600.00909181313.12500133
CCTCATC2100.00400463711.6666663
CTATAAG2850.00341745429.8245621
CTTATTC4905.6474637E-69.2857131
CTATACA3700.00301502138.5135141
CTTACAT4450.00179969447.86516861
TCTTCAG4100.00759323647.68292749
CTTATAA12101.8189894E-117.52066141
CTAAAAG6401.5970925E-47.10937551
CTCTTTC6451.7575585E-47.0542631
GTCATGT8452.0178759E-67.041420513
TGACTTG8803.8138132E-66.76136354
AATCACT5800.00319028766.6379311
CTATAAT5850.00349059246.5811971
GCACACG5850.00349059246.58119768
TGTCATG8652.1416148E-56.47398812
TGATGTC8156.557051E-56.4417189
Produced by FastQC (version 0.11.5)