FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e2-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e2-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420640
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGCG1000.00969196817.49984245
CTATAAG5551.891749E-1011.35244751
AGTCACG3650.00266904738.63005964
ATCTAGG3700.00301605778.5143361
TCCTGAT5401.930548E-58.426742
CTTAATG7553.8255166E-88.3451771
CTAAAGG5109.094157E-58.2360891
CTATTCG3850.00431781548.1826081
CCTATCT3900.00484971248.07770251
CCCGATC7704.6513742E-77.72720351
CCAGTCA4200.0094289627.49993262
CAGTCAC4800.0037855717.291600763
CTATAAT13001.2732926E-117.26993271
CCGATCT8754.5721936E-77.19993552
GCAACCG5904.904247E-47.1185847
CTATTAC5550.0020083166.9376071
ACCCGAT7602.490137E-56.90783250
CATCTCA6652.5592878E-46.84276631
AACCCGA8209.775E-66.829206549
CTCAATG6208.626345E-46.77484751
Produced by FastQC (version 0.11.5)