FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e2-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e2-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511649
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGA6907.358158E-78.116181
GCAATTC4500.0020120067.77774851
TATGACT23250.07.5268532
CTTAACA7502.5856152E-67.46688561
TTCCCTT8059.425239E-77.3912772
CTTTAAG14450.07.26664921
CCCGATC8251.3893095E-67.21209420
GATGTCA16900.07.041393810
CTTATCA10502.6002454E-87.00020551
TGACTTG20500.06.9999744
ATATGAC24700.06.9435231
GTCACGA6056.534641E-46.942122570
TGATGTC16200.06.91355479
GCAACCG5700.00266263546.7543616
AGTCACG5200.00822931256.730966669
ATCCTCT8501.661055E-56.58842851
CTTAAGT5850.00349566226.58138941
ATCCGGT5850.00349687376.58117158
GGAGTCG14401.6007107E-106.56269263
GACTTGA18850.06.4986495
Produced by FastQC (version 0.11.5)