FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e1-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e1-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77298
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGA200.006929157352.49708612
ACCCATG305.846825E-446.66407815
GTTGTGG400.002406192534.9980631
ATGAGTA400.002406192534.9980628
TACCATA450.004285029631.10938638
CCTTTAT500.007148742728.016581
TAAATCT950.007126969818.431964
ATATTTA2000.00272900112.2572541
Produced by FastQC (version 0.11.5)