FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e1-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e1-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856670
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2050.003346727711.9517075
CGGGTGA2050.00334807711.95106362
CCTTACA4753.8111066E-69.57959651
CTTTAAG18500.07.37887761
CTATAAT15750.07.333831
CTATAAG8502.2223812E-67.0004741
CATAGAG6753.0746232E-46.7411971
TATGACT28750.06.69592482
CTCCTCA6803.3645527E-46.69162941
CTATCTA9551.9424206E-66.59730531
CTCTTAA11803.4673576E-86.52586561
CTATATA26600.06.4478051
CTCTAAG7652.0159285E-46.4056631
AGGTTGC6700.0020769376.268574746
CTTACAT10651.7288658E-66.2445541
CTTAGTA7903.056015E-46.2029521
GACTTGA23750.06.1897265
TTACGCC7450.00101829866.10730344
TGACTTG25850.05.957694
CTTAAGA11405.5076835E-65.83372831
Produced by FastQC (version 0.11.5)