FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e1-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e1-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547163
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG850.003788264220.58823654
CTGTCCT900.00527340119.4444453
CGTTGAC1450.00473569514.482758539
ACCAGTT1708.165457E-414.41176560
CCTATAA2102.6479704E-413.3333331
CGTCTGA2600.001593165110.76923170
CTCCAAT2800.00294964210.01
GCACACG3208.0228987E-49.8437565
CTAGTTA2950.00454086539.49152526
CACACGT3450.00159610199.13043466
CTTAATG3200.0088598938.751
CCGCAAC6101.0963831E-68.60655745
ATATAAG6151.2327964E-68.5365851
CGCAACC4905.3285947E-47.85714346
CGATCTT4550.00223709367.69230853
ACCGCAA6502.365968E-57.538461744
GAGTCGA6201.0778855E-47.338710364
GAAGAGC7201.1603246E-57.29166769
GGAGTCG5300.00123082897.264150663
AAGAGCA7251.2790259E-57.241379370
Produced by FastQC (version 0.11.5)