FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e1-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e1-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181405
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGG750.001833555923.33333459
GTACGGT1951.4152689E-414.35897443
CGCACCC2305.7177304E-412.17391346
GCAACCG7655.2114046E-98.69281147
GTCATGT28450.08.48857613
AGCAGAC4159.045964E-48.43373560
GATGTCA25950.08.36223510
CGCAACC19800.08.30808146
TCATGTG36700.08.29700314
TGATGTC25750.08.2912629
TATGACT35750.08.2237762
TGTCATG30450.08.1609212
CATGTGT38700.08.13953515
ATATGAC37100.07.92452861
GTGTATG38000.07.92105318
TTCCGAG4905.347406E-47.85714311
TGACTTG31750.07.82677174
GAGTATA39950.07.797246528
GACTTGA28750.07.7913045
ACCGCAA28350.07.654321744
Produced by FastQC (version 0.11.5)