FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_UB_e1-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_UB_e1-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263817
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGGG850.003790449120.58872432
CCGCTAT1450.00474074714.4825349
CTCAGAG3351.05509265E-410.4480091
CGCAACC9850.09.23843246
GCAACCG4057.095149E-48.64183947
CCGCAAC12500.07.839875745
TGACTTG17500.07.59987974
ATATGAC19650.07.48109341
TATGACT19200.07.47383982
CTTTAAG13202.0008883E-117.1592611
CTATTGA7351.557355E-57.1430271
GACTTGA16850.07.06220345
CTATTAC5955.3980213E-47.05899051
CTTATAG6501.9358628E-47.0001661
CAACCCG5500.00182708916.999889448
ACCCGAT6157.87519E-46.829160750
ATGACTT19050.06.79779243
CGGGTTG7301.0959373E-46.712222617
GGGTTGC6904.020601E-46.594098618
CTTAAAG13301.2423698E-96.5791041
Produced by FastQC (version 0.11.5)