FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e3-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e3-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29998
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA150.002203749570.000014
ACAATTG150.002203749570.0000157
AAGTATC200.00688644152.524
TTGCAAC200.00688644152.534
CTCCAAT200.00688644152.51
CTCGTAA200.00688644152.563
TAAAGTA350.001236292240.022
AAAGTAT458.989045E-538.88888523
ACTAAAT450.004242439831.11110910
TACAAAT450.004242439831.11110921
CTAAATA450.004242439831.11110911
CAACTTT500.00710054528.00000237
TTACAAT500.00710054528.00000255
AATTTTA850.003706500220.58823658
Produced by FastQC (version 0.11.5)