FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e3-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e3-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386828
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA150.00222274370.008285
CGCGGAC150.002223882869.9992223
TCGAGCG150.002223882869.9992214
CCTACCG252.3891778E-455.99938640
CTACCGG252.3891778E-455.99938641
GGCGCTA452.6948328E-854.450884
CGGCGCT200.0069453252.5062073
TACGGCG200.0069453252.5062071
ACGGCGC200.0069453252.5062072
GCGCTAT200.0069453252.5062075
ATGGCGC200.00694887252.49941641
CCTAGGG200.00694887252.4994167
GCTGGGA200.00694887252.49941648
GCGCGTC200.00694887252.4994168
CCCGTGA200.00694887252.49941654
CGCTCTG200.00694887252.49941645
ACTAGCG200.00694887252.49941633
GCGTGCG200.00694887252.49941653
GGCGAGC200.00694887252.49941620
GCGCTCC200.00694887252.49941664
Produced by FastQC (version 0.11.5)