FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e3-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e3-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271566
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA150.002223069570.000014
CGTCTCA308.362938E-658.33333661
GGATACG252.3877753E-455.9999968
GCCCTCA252.3877753E-455.99999643
CGGGTAC200.00694634952.553
GGTACGG200.00694634952.555
GGGTACG200.00694634952.554
GGTCCAG200.00694634952.555
TCGGGCA200.00694634952.557
GCTGCAC200.00694634952.55
CGTTCGA200.00694634952.561
AGCGGAG200.00694634952.554
GCGCTAG200.00694634952.55
GCTCGAC200.00694634952.522
ACGAGGG200.00694634952.516
GCCCGTT200.00694634952.532
GTACGGG200.00694634952.556
GAGGCTA200.00694634952.570
AGGGTAG352.0832262E-550.00000457
CGGAGAG352.0832262E-550.00000463
Produced by FastQC (version 0.11.5)