FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e3-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e3-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246547
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCG207.916515E-569.99959620
CCCGTAG150.002222877569.9995968
GTATGCG150.002222877569.9995947
GTGCGGT150.002222877569.9995911
CGAAGCC150.002222877569.9995917
ACGCTAG252.3874099E-455.99967628
CGGCTCA252.3874099E-455.99967621
GCCCGCC252.3874099E-455.99967622
GTACGGT200.006940181452.5103452
GATCGCT200.006945752552.49969519
CCAGGTC200.006945752552.49969533
GCGAAGG200.006945752552.49969561
GTAGGGG200.006945752552.49969564
GCCGCAT200.006945752552.49969549
AGCGCGA200.006945752552.49969566
TAGGGGC200.006945752552.49969565
ACCCGGA200.006945752552.49969525
CGGTAAC200.006945752552.49969537
TGCCGCC200.006945752552.49969559
CCCGAGC200.006945752552.49969540
Produced by FastQC (version 0.11.5)