FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e3-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e3-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300233
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAATG150.00222329970.0000114
TAACGGG150.00222329970.0000140
GCGCAGT150.00222329970.0000118
CGAGGCG150.00222329970.0000155
GTCCGAT150.00222329970.0000148
TAGGCGG150.00222329970.0000128
GACGCCA150.00222329970.0000137
GTCCAGG150.00222329970.000012
GCGTACG150.00222329970.000016
GCGAGGA252.388183E-455.99999626
CTGCTGG200.0069470652.512
GAGGACC200.0069470652.564
GGGCTAG200.0069470652.560
TGCGGCA200.0069470652.522
TCGGCGA200.0069470652.565
ACGATGG200.0069470652.518
GAGACGT200.0069470652.56
GCCTCGC200.0069470652.519
AGGGGCA200.0069470652.511
CCTGACC200.0069470652.522
Produced by FastQC (version 0.11.5)