FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e2-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e2-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17735
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTTA150.002188789770.0000116
TTTTGAC200.006840049852.50000441
GTTCTTT200.006840049852.50000445
AATAAAC200.006840049852.5000047
TTGCTTT200.006840049852.50000416
ATGAGAA200.006840049852.50000462
TTAAAAA500.007023180428.0000023
Produced by FastQC (version 0.11.5)