FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e2-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e2-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333289
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG150.002223514570.041
CGTGTCG207.920371E-570.055
CCGGCTA252.3885662E-455.99999655
GACACCC200.00694772852.510
CGGCACT200.00694772852.522
GACCCGT200.00694772852.563
ACTCGCA200.00694772852.544
GCGGCAC200.00694772852.521
GGCGCAT200.00694772852.564
CGCGTCT200.00694772852.549
ATCGGCC200.00694772852.57
CCCGGTG200.00694772852.569
CCCGGGG200.00694772852.548
CGCCCGG200.00694772852.546
CGCGGAT352.0842566E-550.037
TGTGCGA305.8733625E-446.6666649
CGAAGTG305.8733625E-446.66666414
AACGCGG404.5877514E-543.7535
CGGCGCC350.001254486440.011
GATCGCT350.001254486440.051
Produced by FastQC (version 0.11.5)