FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e2-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e2-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214350
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGG150.002222429570.039
GTAGCTC252.3866363E-455.99999657
CGTCGGA252.3866363E-455.99999625
ACGACTG252.3866363E-455.99999664
CGGCATA200.00694436252.538
GCTGCCG200.00694436252.555
GGTCAGG200.00694436252.522
GCCGCTA200.00694436252.54
CGGGAGC200.00694436252.543
CACAGGC200.00694436252.528
CGCACGA200.00694436252.567
ACTCCGG200.00694436252.550
TCCCACC200.00694436252.553
GTCGGGA200.00694436252.541
CGCTAGA352.081743E-550.06
AGTCCGA305.868647E-446.66666421
GTCGACG305.868647E-446.66666446
CGGCATT305.868647E-446.66666436
CTGCCGT305.868647E-446.66666456
GGTCCTA350.001253485340.04
Produced by FastQC (version 0.11.5)