FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e2-1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e2-1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296831
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCA150.002223401369.99898520
GCGGCTA150.002223401369.99898510
GACGCGG150.002223401369.99898518
ACGCGGC150.002223401369.99898519
CGGCCTT252.388309E-455.999199
CATGCGC200.0069473852.4992459
CCGCTCA200.0069473852.4992441
TGCGCTG200.0069473852.4992461
CGCTCGG200.0069473852.4992464
GCTCGTC200.0069473852.4992436
CCGTTAG200.0069473852.4992432
CGCTAGA305.8678596E-446.6738556
CTAGTGC305.8727356E-446.66599363
GAGCCCG305.8727356E-446.66599356
CCGAAGC305.8727356E-446.66599350
GTCCTCA503.46846E-642.006475
GGGGTGC350.001254353839.9994213
ACTACCG350.001254353839.9994255
TCGCACA459.186192E-538.8883364
GACAGCA501.705888E-435.005396
Produced by FastQC (version 0.11.5)