FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e2-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e2-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352002
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGG150.002223636569.99985559
GTCGGGG150.002223636569.99985560
AGCCGCT200.006948105552.49989768
CGCGAGG200.006948105552.49989769
AGGCAGC200.006948105552.49989714
CGCTAGA200.006948105552.4998976
TCGTCAG200.006948105552.49989757
GCGGACG200.006948105552.49989746
CGCCCGG200.006948105552.49989746
ACGACCG200.006948105552.49989762
GGGTCCT850.049.4116633
GTCATCC305.873874E-446.66657361
GCGTTGC305.873874E-446.6665739
GTTAGCG350.001254595139.9999224
AGTAGCG350.001254595139.9999232
CGCAAGC350.001254595139.9999253
CTCGGTG459.189006E-538.8888126
GCTGGTG400.002417184934.9999350
CGCATCG400.002417184934.9999339
CAGCACT400.002417184934.9999340
Produced by FastQC (version 0.11.5)