FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e1-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e1-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217414
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCC200.006944494352.563
TCGACAG200.006944494352.532
GTGGGTG400.00241513535.057
ACAGGGG500.00719771528.00000247
CTGGGAC500.00719771528.00000249
ACCACTT1200.001336333217.553
CTAAGAT1554.0363113E-415.8064521
CTCCTCA1450.00472352914.48275851
CAATATA3200.0088243178.751
ATATAAT7300.0053500185.75342461
ATATATT8558.303641E-45.7309941
Produced by FastQC (version 0.11.5)