FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e1-1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e1-1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551157
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGCG150.00222428870.0000164
CGGGGGC200.006950127452.568
GTAAGGC400.002418344835.032
CAGCTCG750.001831826223.33333415
ATTCCCC950.00720595918.4210537
CTATAGG1503.1554032E-416.3333341
GGCAGAA1600.00908821213.12510
AGGAGAT2802.3399002E-411.251
GGTTTGA3752.9059041E-510.26666558
AATTGTC3400.0013972069.2647061
CTTATAA5452.2255772E-68.9908271
CTATTGT3600.00235036768.7500011
CTCTTTG3650.00266349068.63013651
TACGAGT3900.0048443718.07692329
GTTAAAT5159.069829E-47.47572852
ATCATCT4700.00307437947.4468091
ATTAGTT6602.3272283E-46.89393951
ATCATCA7351.1930897E-46.6666671
CTATATA9702.4912752E-66.49484541
ATTATGT6700.00206913246.26865671
Produced by FastQC (version 0.11.5)