FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_ColSPL9_Col_B_e1-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_ColSPL9_Col_B_e1-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310675
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGGG305.872811E-446.6665640
GTCCTTT2651.3965706E-411.8886791
TAATTTC3100.00681587369.0322376
GATATAA3100.00681587369.03223723
ATATATG6907.864037E-88.6245571
ATATGAA6201.0705841E-47.3398741
TATATGA5100.00678714236.86272952
ATATGTA7209.04359E-56.8066361
TATATGT6650.0018943916.31577542
Produced by FastQC (version 0.11.5)