FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_C24SPL9_C24_B_e-1i.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_C24SPL9_C24_B_e-1i.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34777
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTCCACCTGGCGGAAGCAGGTGTCGAGCAGCGCCTTCGTCGTCGACG480.13802225608879432No Hit
CCCTGAAAAGGAGGTGATCCAGCCGCACCTTCCAGTACGGCTACCTTGTT350.1006412283980792No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCT150.00220674370.040
GGCACGA150.00220674370.014
AACTTCG150.00220674370.069
GCACGAT207.8210905E-570.015
TCGGCAC150.00220674370.012
ACAGGTC150.00220674370.036
GTGTCAA150.00220674370.057
ACATGGT150.00220674370.049
TTCATTG150.00220674370.063
TTCGCTT150.00220674370.056
AATTCGA252.3588043E-455.99999627
CATGGTT200.00689572252.550
ATGGGTC200.00689572252.51
CTTCGAA200.00689572252.534
AACAACC200.00689572252.561
TCTGAAA200.00689572252.530
GTCCTAT200.00689572252.55
CGGCACG200.00689572252.513
TTCAATG200.00689572252.550
CACGATA200.00689572252.516
Produced by FastQC (version 0.11.5)