FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_C24SPL9_C24_B_e-1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_C24SPL9_C24_B_e-1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817652
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTCCACCTGGCGGAAGCAGGTGTCGAGCAGCGCCTTCGTCGTCGACG8300.10151017792410463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCCT1100.057.27272456
TGCCGGG900.054.44444346
GGGTGCG1400.052.561
TGCGGCA1050.050.00000464
CACGCGT707.2759576E-1250.030
ACCGCGC850.049.41176636
CCGCGCG850.049.41176637
GCGCTTA850.049.4117661
GCGCTGA1350.049.25926263
GCCGGGC1000.049.00000447
GCGCTAT506.19857E-849.00000435
CGCGAGT1350.046.66666846
CCTGACG305.8784016E-446.66666817
CGCGCGA900.046.66666438
CAGCGCC1450.045.8620731
CACGCCA1150.045.65217248
TGAGGCG1000.045.50000439
GGGCCCA851.8189894E-1245.29411736
CGCCAGC851.8189894E-1245.29411711
GCCTTCG1650.044.54545635
Produced by FastQC (version 0.11.5)