FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n01_C24SPL9_C24_B_e-1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n01_C24SPL9_C24_B_e-1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818623
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGG650.059.23076639
GCGCCTG308.377947E-658.33333217
TGCGCCT308.377947E-658.33333220
AACGCGG252.390629E-456.00000448
CGACCGC452.7022907E-854.44444312
TGCGCGT850.053.529411
GGCAGGC200.00695132652.511
GGCCCTC200.00695132652.555
TGCGCGG407.4816853E-752.529
GGCTCGC552.2864697E-950.90909625
CCGTGCG352.0869455E-550.00000434
GCCGGGC506.19857E-849.00000447
GGGTCCT1300.048.4615363
ACCGCGC305.878407E-446.66666436
GCGCACT305.878407E-446.6666647
AGGGGGC305.878407E-446.66666459
GCGCCCT703.8380676E-1045.00000456
CGGTGGG551.3120007E-744.54545616
TCCGCGA404.5936424E-543.7527
GCGCGCC404.5936424E-543.7515
Produced by FastQC (version 0.11.5)